LIpHOSOME for dosing methods

Over the past 50 years, numerous methods have been developed for the in vitro detection of diagnostic markers in biological fluids. These analyzes often rely on antigen / antibody recognition (immunoassay). However, there is a great need to develop methods with higher sensitivity, lower costs and higher semplicity to be performed.
The present invention relates to a composition of liposomes containing an aqueous solution at a different pH with respect to the pH of the solution in which they are suspended. Such a liposome has at least one specific ligand on the surface for an analyte of interest contained in a biological fluid. This feature allows exploiting this system in dosing methods.The presence of the liposome, possibly linked to the ligand of interest, is highlighted through a detectable pH variation by means of a pH meter or, more banally, an indicators or litmus paper. The method has the advantage over existing ones of using low-cost instrumentations available to any scientific laboratory (pH meter). A dosing method has been proposed and called LICIA (LIpHosomes Conjugated Immunosorbent Assay).

• Reduction of preparation costs
• Easy use by unskilled personnel
• Use of low-cost equipment in any laboratory
• Wide method versatility: The LipHosoma can be functionalized to detect different targets in various diagnostic kits

Filing date: 24/05/2019

Application number: 102019000007222